Construction of a SSR-based genetic map and identification of QTLs for catechins content in tea plant (Camellia sinensis).

作者: Jian-Qiang Ma , Ming-Zhe Yao , Chun-Lei Ma , Xin-Chao Wang , Ji-Qiang Jin

DOI: 10.1371/JOURNAL.PONE.0093131

关键词:

摘要: Catechins are the most important bioactive compounds in tea, and have been demonstrated to possess a wide variety of pharmacological activities. To characterize quantitative trait loci (QTLs) for catechins content tender shoots tea plant, we constructed moderately saturated genetic map using 406 simple sequence repeat (SSR) markers, based on pseudo-testcross population 183 individuals derived from an intraspecific cross two Camellia sinensis varieties with diverse composition. The consisted fifteen linkage groups (LGs), corresponding haploid chromosome number plant (2n = 2x 30). total length was 1,143.5 cM, average locus spacing 2.9 cM. A 25 QTLs associated were identified over measurement years. Of these, nine stable validated across years, clustered into four main regions LG03, LG11, LG12 LG15. variability explained by each QTL predominantly at moderate-to-high levels ranged 2.4% 71.0%, 17.7%. varied eight, while all between 38.4% 79.7%. This is first report identification plant. results this study provide foundation further cloning functional characterization catechin utilization improvement

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