Delayed DNA replication in haploid human embryonic stem cells
作者： Benvenisty N , Ding Q , Koren A , Egli D , Zuccaro Mv
DOI: 10.1101/2021.05.11.443666
关键词:
摘要: Haploid human embryonic stem cells (ESCs) provide a powerful genetic system but diploidize at high rates. We hypothesized that diploidization results from aberrant DNA replication. To test this, we profiled replication timing in isogenic haploid and diploid ESCs. The greatest difference was the earlier of X chromosome haploids, consistent with lack inactivation. Surprisingly, also identified 21 autosomal regions had dramatically delayed extending beyond normal S phase into G2/M. Haploid-delays comprised unique set quiescent genomic are under-replicated polyploid placental cells. same delays were observed female ESCs two active chromosomes, suggesting increased dosage may cause propose incomplete onset mitosis could prevent cell division result re-entry cycle whole genome duplication. HighlightsO_LIDNA by WGS C_LIO_LIExtreme C_LIO_LIReplication associate X-chromosome multiple systems correspond to underreplication mouse C_LI
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