AAV Delivery of Endothelin-1 shRNA Attenuates Cold-Induced Hypertension.
作者： Peter Gin-Fu Chen , Zhongjie Sun , None
DOI: 10.1089/HUM.2016.047
关键词:
摘要: Cold temperatures are associated with increased prevalence of hypertension. exposure increases endothelin-1 (ET1) production. The purpose this study is to determine whether upregulation ET1 contributes cold-induced hypertension (CIH). In vivo RNAi silencing the gene was achieved by adeno-associated virus 2 (AAV2) delivery short-hairpin small interfering RNA (ET1-shRNA). Four groups male rats were used. Three given AAV.ET1-shRNA, AAV.SC-shRNA (scrambled shRNA), and phosphate-buffered saline (PBS), respectively, before a moderately cold environment (6.7 ± 2°C), while last group PBS kept at room temperature (warm, 24 ± 2°C) served as control. We found that systolic blood pressure PBS-treated SC-shRNA–treated significantly within weeks cold, reached peak level (145 ± 4.8 mmHg) 6 weeks, remained elevated thereafter. By contrast, ET1-shRNA-treated did n...
sci-hub.se 参考文章(48)
Agustín Lara Esqueda, Gustavo Pastelín Hernández, Martín Rosas Peralta, Fause Attie, Grupo Encuesta Nacional de Salud, Oscar Velázquez Monroy, Roberto Tapia Conyer, [Arterial hypertension in Mexico: results of the National Health Survey 2000]. Revista Portuguesa De Pneumologia. ,vol. 72, pp. 71- 84 ,(2002)
K. A. HIGH, Gene therapy for haemophilia: a long and winding road. Journal of Thrombosis and Haemostasis. ,vol. 9, pp. 2- 11 ,(2011) , 10.1111/J.1538-7836.2011.04369.X
Z. SUN, J. R. CADE, M. J. FREGLY, Cold‐Induced Hypertension Annals of the New York Academy of Sciences. ,vol. 813, pp. 682- 688 ,(1997) , 10.1111/J.1749-6632.1997.TB51767.X
Yi Lin, Zhongjie Sun, Antiaging Gene Klotho Attenuates Pancreatic β-Cell Apoptosis in Type 1 Diabetes Diabetes. ,vol. 64, pp. 4298- 4311 ,(2015) , 10.2337/DB15-0066
Kai Chen, Xiaoli Zhou, Zhongjie Sun, Haplodeficiency of Klotho Gene Causes Arterial Stiffening via Upregulation of Scleraxis Expression and Induction of Autophagy Hypertension. ,vol. 66, pp. 1006- 1013 ,(2015) , 10.1161/HYPERTENSIONAHA.115.06033
R F Heller, G Rose, H D Pedoe, D G Christie, Blood pressure measurement in the United Kingdom Heart Disease Prevention Project. Journal of Epidemiology & Community Health. ,vol. 32, pp. 235- 238 ,(1978) , 10.1136/JECH.32.4.235
Xiuqing Wang, Lucille Skelley, Bo Wang, Ayesha Mejia, Val Sapozhnikov, Zhongjie Sun, AAV-Based RNAi Silencing of NADPH Oxidase gp91phox Attenuates Cold-Induced Cardiovascular Dysfunction Human Gene Therapy. ,vol. 23, pp. 1016- 1026 ,(2012) , 10.1089/HUM.2012.078
M. Ian Phillips, Yi Tang, Kai Schmidt-Ott, Keping Qian, Shuntaro Kagiyama, Vigilant vector: Heart-specific promoter in an adeno-associated virus vector for cardioprotection Hypertension. ,vol. 39, pp. 651- 655 ,(2002) , 10.1161/HY0202.103472
ZHONGJIE SUN, MELVIN J FREGLY, NEIL E ROWLAND, J.ROBERT CADE, Comparison of Changes in Blood Pressure and Dipsogenic Responsiveness to Angiotensin II in Male and Female Rats Chronically Exposed to Cold Physiology & Behavior. ,vol. 60, pp. 1543- 1549 ,(1996) , 10.1016/S0031-9384(96)00325-3
Z. Sun, R. Cade, C. Morales, Role of central angiotensin II receptors in cold-induced hypertension. American Journal of Hypertension. ,vol. 15, pp. 85- 92 ,(2002) , 10.1016/S0895-7061(01)02230-0