Comparative transcriptome analysis to investigate the high starch accumulation of duckweed (Landoltia punctata) under nutrient starvation.

摘要: Background: Duckweed can thrive on anthropogenic wastewater and produce tremendous biomass production. Due to its relatively high starch low lignin percentage, duckweed is a good candidate for bioethanol fermentation. Previous studies have observed that water devoid of nutrients accumulation, but molecular mechanism remains unrevealed. Results: This study globally analyzed the response nutrient starvation in order investigate accumulation (Landoltia punctata). L. punctata was transferred from nutrient-rich solution distilled sampled at different time points. Physiological measurements demonstrated activity ADP-glucose pyrophosphorylase, key enzyme synthesis, as well percentage duckweed, increased continuously under starvation. Samples collected 0 h, 2 h 24 points respectively were used comparative gene expression analysis using RNA-Seq. A comprehensive transcriptome, comprising 74,797 contigs, constructed by de novo assembly RNA-Seq reads. Gene profiling results showed some transcripts encoding enzymes involved biosynthesis up-regulated, while consumption down-regulated, photosynthesis-related down-regulated during first transporter up-regulated within h. Very interestingly, most flavonoid highly expressed regardless starvation, laccase, last rate-limiting lignifications, exhibited very abundance all three samples. Conclusion: Our provides which indicates global metabolic status, redirects flux fixed CO2 into synthesis branch resulting punctata.