Transplantation of Adipose Stromal Cell Sheet Producing Hepatocyte Growth Factor Induces Pleiotropic Effect in Ischemic Skeletal Muscle

摘要: Cell therapy remains a promising approach for the treatment of cardiovascular diseases. In this regard, contemporary trend is development methods to overcome low cell viability and enhance their regenerative potential. present study, we evaluated therapeutic potential gene-modified adipose-derived stromal cells (ADSC) that overexpress hepatocyte growth factor (HGF) in mice hind limb ischemia model. Angiogenic neuroprotective effects were assessed following ADSC transplantation suspension or form sheet. We found superior blood flow restoration, tissue vascularization innervation, fibrosis reduction after HGF-producing sheet compared other groups. suggest observed are determined by pleiotropic HGF, along with multifactorial paracrine action which remain viable functionally active within engineered construct. Thus, demonstrated high utilized skeletal muscle recovery ischemic damage associated complex degenerative effects.