A specific radioimmunoassay of plasma progesterone.

摘要: ABSTRACT A specific progesterone antibody was obtained by immunizing a ewe with 11α-hydroxy-progesterone-hemisuccinate conjugated to bovine serum albumin. This used for the assay of in crude plasma extracts. The sensitivity 0.05 ng. accuracy confirmed recovery known amounts added plasma. inter- and intra-assay coefficient variation 8%. Specificity a) checking cross reactivity various steroids; b) failure other steroids influence accurate measurement progesterone, c) finding comparable levels extracts without chromatography. Mean values (ng/ml) were 0.26 ± 0.08 (sd) males postmenopausal females; 0.50 0.44 preovulatory 12.1 4.81 postovulatory females.