Antibody-binding sites on truncated forms of varicella-zoster virus gpI(gE) glycoprotein

摘要: Varicella-zoster virus (VZV)-seropositive human sera were shown to be reactive with the truncated VZV gp1(gE) candidate subunit vaccine (TgpI-511). To identify location of antibody-binding sites (epitopes) on TgpI-511, three forms TgpI-511 glycoprotein (TgpI-124, TgpI-160, TgpI-316) DNA encoding N-terminal region this amino acid residues 124, 160 and 360, respectively, inserted into vaccinia genome. Infection cells recombinant viruses resulted in secretion all gpI(gE) as well from infected cells. Immunoprecipitation these glycoproteins VZV-seropositive gpI(gE)-speck monoclonal antibodies identified four new glycoprotein. In addition, tunicamycin treatment O-glycanase digestion revealed presence both N-linked O-linked oligosaccharides TgpI-511. These results epitopes demonstrated that recognized by individuals.