Influence of Lactoferrin on Host-Microbe Interactions

摘要: The antimicrobial spectrum of lactoferrin (Lf) includes statsis, cidal, cationic, phagocytic, and colonization/decolonization effects on susceptible microorganisms. selective interaction Lf with microbial surface seems to play an essential role in regulating many these events. bound specific outer membrane (OM) pore-forming proteins (porins) various enteric bacteria. magnitude Lf-microbe showed a direct relationship the bacterial susceptibility Lf. Although most bacteria expressed porins, certain strains resistance did not demonstrate binding. This “resistance” was attributed shielding porin accessibility by carbohydrate O-antigenic chains lipopolysaccharide (LPS). Mutants progressive deletions O-side-chain core polysaccharide demonstrated increased binding effects. affected channels OM potentiated diffusion antibiotics. also intestinal colonization Escherichia coli. inhibited expression fimbriae blocked host subepithelial matrix components, such as fibronectin, fibrinogen, collagens, laminin. Our studies suggested that cell target involved anion-binding ligands Lf, followed coordinate oxidation Fe2+ Fe3+ surface. metal transition presence suitable oxygen species fulfill necessary components Haber-Weiss reaction promote targeted radical generation, iron (Fe) serving prosthetic group