A stable method for routine analysis of oxylipins from dried blood spots using ultra-high performance liquid chromatography-tandem mass spectrometry.

摘要: Summary Oxylipins are biologically important lipid mediators that derived enzymatically from polyunsaturated fatty acids (PUFA) and have a major role in regulating inflammatory processes. The currently available methods for measuring oxylipins human biological samples limitations, which restricts their use large studies. We developed novel method 21 dried blood spot (DBS) using ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) stable isotope dilution analysis. Our new is reproducible precise enables the high throughput analysis quantitation of bioactive small volumes blood. In future, this can be readily applied to measure Abstract downstream enzymatically-produced implicated as effectors these acids. Recently reported require complex extraction procedures. study, we report development validation system individual (UHPLC–MS/MS) Linearity precision were determined stabilities 12 most abundant tested during 2 months storage at room temperature, after being spiked into prepared DBS on PUFAcoat™ paper. Responses linear across concentration range analysed all (r2 values ranged 0.953 0.998). Intra–day inter–day variations were ≤16% oxylipins. Recovery 80 – 115%. when stored temperature. precise, provides opportunity accurately quantitate sample volume.