Removal of fibroblasts from human epithelial cell cultures with use of a complement fixing monoclonal antibody reactive with human fibroblasts and monocytes/macrophages.
作者： Kay H. Singer , Richard M. Scearce , Debbi T. Tuck , Leona P. Whichard , Stephen M. Denning
DOI: 10.1111/1523-1747.EP12276685
关键词:
摘要: A complement fixing IgM monoclonal antibody (1B10) that reacts with surface membrane molecules of human fibroblasts, tissue macrophages, and peripheral monocytes was produced. In Western blot analysis detergent extracts cultured foreskin 1B10 detected protein bands Mr 43,000 72-80,000. We used the to eliminate most positive nonepithelial cells from thymic epithelial (TE) cell cultures, thereby allowing us grow highly enriched populations TE cells.
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