Quantification of hurdles: predicting the combination of effects -- Interaction vs. non-interaction.

摘要: Combination of disparate as well related antimicrobial effects constitutes the concept hurdle technology. Quantification combined effects, including claims synergy, can be accomplished using surface response modelling, is frequently done and reported. The Gamma hypothesis, however, states that relative different factors combine independently. Studies performed time to detection have shown hypothesis an adequate foundation for analysis multi-factor environmental stresses placed on microorganisms, pH, weak acids temperature. Data from action Na acetate pH Aeromonas hydrophila, acetate/pH , K sorbate/pH acetate/K sorbate at 6.5, 6.0 5.5 Escherichia coli temperature Enterobacter sakazakii were examined nominal logistic modelling (RS) by a model. model used in predictive manner unlike RS models parameters approximated fit observed data. expansion explains occurrence statistically significant cross terms polynomials. emphasis within literature seeking interactions or synergies between should replaced with one emphasising falsification approach. This examining ratios gamma when combination, but this also requires use appropriate functions do this.