Ursolic acid induces cell cycle arrest and apoptosis of gallbladder carcinoma cells

摘要: Ursolic acid (UA), a plant extract used in traditional Chinese medicine, exhibits potential anticancer effects various human cancer cell lines vitro. In the present study, we evaluated anti-tumoral properties of UA against gallbladder carcinoma and investigated mechanisms responsible for its on proliferation, cycle arrest apoptosis The anti-tumor activity GBC-SD SGC-996 cells was assessed using MTT colony formation assays. An annexin V/PI double-staining assay to detect apoptosis. Cell changes were detected flow cytometry. Rhodamine 123 staining assess mitochondrial membrane (ΔΨm) validate UA’s ability induce both lines. effectiveness further verified vivo by establishing xenograft GBC model nude mice. Finally, expression levels cycle- apoptosis-related proteins analyzed western blotting. Our results suggest that can significantly inhibit growth cells. assays indicated dose-dependent decreases proliferation. S-phase observed after treatment with UA. Annexin suggested induced early late phases also decreased ΔΨm altered molecules regulating study showed intraperitoneally injection inhibited tumor mice inhibition efficiency is dose related. Activation caspase-3,-9 PARP pathways may be involved UA-induced Taken together, these significant suppressing promoting inducing 7cell vitro vivo. It agent treating cancer.