Green fluorescent protein as a quantitative reporter of relative promoter activity in E. coli.

摘要: Green fluorescent protein (GFP) has become a valuable tool for the detection of gene expression in prokaryotes and eukaryotes. To evaluate its potential quantitation relative promoter activity E. coli, we have compared GFP with commonly used reporter lacZ, encoding beta-galactosidase. We cloned series previously characterized synthetic coli promoters into beta-galactosidase vectors. Qualitative quantitative assessments these constructs show that (a) both reporters display similar sensitivities cells grown on solid or liquid media (b) is especially well suited agar. Thus, provides simple, rapid sensitive measuring intact cells.