Cochaperone Binding to LYR Motifs Confers Specificity of Iron Sulfur Cluster Delivery

摘要: Iron sulfur (Fe-S) clusters, preassembled on the ISCU scaffold, are transferred to target proteins or intermediate scaffolds by a dedicated chaperone-cochaperone system. However, molecular mechanisms that underlie substrate discrimination and guide delivery of nascent clusters specific subsets Fe-S recipients poorly understood. Here, we identified interacting partners cochaperone HSC20 discovered LYR motifs signatures recipient accessory factors assist cluster delivery. In succinate dehydrogenase B, two engage ISCU-HSC20-HSPA9 complex aid incorporation three within final structure II. Moreover, show members motif family which assembly complexes II III, SDHAF1 LYRM7, respectively, binding partners. Our studies unveil network interactions between motif-containing key function I, II, III.