Ultrahigh-Throughput Improvement and Discovery of Enzymes Using Droplet-Based Microfluidic Screening
作者： Alexis Autour , Michael Ryckelynck
DOI: 10.3390/MI8040128
关键词:
摘要: Enzymes are extremely valuable tools for industrial, environmental, and biotechnological applications there is a constant need improving existing biological catalysts discovering new ones. Screening microbe or gene libraries an efficient way of identifying enzymes. In this view, droplet-based microfluidics appears to be one the most powerful approaches as it allows inexpensive screenings in well-controlled conditions ultrahigh-throughput regime. This review aims introduce main microfluidic devices concepts considered such screening before presenting discussing latest successful technology enzyme discovery.
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