Preferential signal production on a surface in immunoassays

作者: Edwin F. Ullman , David J. Litman

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摘要: PREFERENTIAL SIGNAL PRODUCTION ON A TEST STRIP IN IMMUNOASSAYS ABSTRACT OF THE DISCLOSURE An assay method and compositions are provided for determining the presence of an analyte in a sample. The is member immunological pair (mip) immunogens--ligand receptor. has two basic elements: solid surface to which one members bonded signal producing system, includes catalytic mip, system results measurable on said related amount medium. generating compound produced without separation catalyst labeled mip bound from free solution. In preferred embodiment, enzyme acts conjunction with solute produce product binds preferentially when surface, resulting in-a readily differentiated by bulk

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