Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells.

摘要: Abstract Mutant lines of Chinese hamster ovary cells that show hypersensitivity to killing and mutagenesis by UV light were analyzed genetic complementation analysis determine whether defects in different gene loci might underlie a common cellular phenotype,. To facilitate rapid screening mutant clones, procedure was devised allowed presumptive be assessed on the basis frequency UV-resistant after fusion polyethylene glycol. Four classes identified among 44 clones tested. By using drug-resistance markers for selection hybrid crosses between wild type, from each four shown behave as phenotypically recessive. Hybrids also isolated pair combinations mutants. All such hybrids relatively resistant killing, providing confirmation classes. When mutants representing UV-complementation tested with polyaromatic hydrocarbon 7-bromomethylbenz(a)anthracene, again seen all combinations. These results suggest class represents biochemical defect plays role repair both UV-induced chemically induced lesions DNA.