Development of HA/Ag-NPs Composite Coating from Green Process for Hip Applications.
作者： Denisse Lozoya-Rodríguez , Renata de Lima , Leonardo Fraceto , Antonio Ledezma Pérez , Mercedes Bazaldua Domínguez
DOI: 10.3390/MOLECULES22081291
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摘要: In the present study, biological hydroxyapatite (HA) was obtained from bovine bones through a thermal process. A total of 0% and 1% silver nanoparticles (Ag-NPs) synthesized Opuntia ficus (nopal) were added to coatings using an atmospheric plasma spray (APS) on Ti6Al4V substrate. Following this, its antimicrobial efficiency evaluated against following bacterial strains: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa. This conducted according Japanese Industrial Standard (JIS) Z2801:2000 “Antimicrobial Product-Test for Antimicrobial Activity Efficacy”. Scanning electron microscopy (SEM) showed that evenly distributed coating surface. Energy dispersive X-ray spectroscopy (EDX) shows apatite deposition occurs daily basis, maintaining Ca/P rate between 2.12 1.45. Biocompatibility properties with osteoblast-like cells (MC3T3-E1) by single-cell gel electrophoresis assay Tali image cytometry.
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