Cross-linked enzyme aggregates of arylamidase from Cupriavidus oxalaticus ICTDB921: process optimization, characterization, and application for mitigation of acrylamide in industrial wastewater

摘要: Acrylamidase produced by Cupriavidus oxalaticus ICTDB921 was recovered directly from the fermentation broth ammonium sulfate (40–50%) precipitation and then stabilized cross-linking with glutaraldehyde. The optimum conditions for preparation of cross-linked enzyme aggregates acrylamidase (acrylamidase-CLEAs) were using 60 mM glutaraldehyde 10 min at 35 °C initial pH 7.0. Acrylamidase-CLEAs characterized SDS-PAGE, FTIR, particle size analyzer SEM. Cross-linking shifted optimal temperature 70 to 50 °C 5–7 6–8, respectively. It also altered secondary structure fractions, thermal stability along kinetic constants, Km Vmax, A complete degradation acrylamide ~ 1.75 g/L in industrial wastewater achieved after 60 min a batch process under operating conditions, kinetics best represented Edward model (R2 = 0.70). retained ~ 40% its activity three cycles both pure acrylamide wastewater, stable 15 days 4 °C, retaining ~ 25% original activity.