1,25-Dihydroxyvitamin D3 modulates bone marrow macrophage precursor proliferation and differentiation. Up-regulation of the mannose receptor.

摘要: Abstract 1,25-Dihydroxyvitamin D3 (1,25-(OH)2D3), the biologically active form of vitamin D3, has been shown to inhibit proliferation and promote monocytic differentiation leukemic cell lines. In present communication, we extend these observations normal bone marrow macrophage precursors, 1) identify stage maturation wherein steroid exerts its antiproliferative effect, 2) demonstrate that 1,25-(OH)2D3 promotes as manifest by specific up-regulation lineage-specific membrane protein, mannose-fucose receptor. experiments, 1,25-(OH)2D3-mediated inhibitory effect on colony formation was be independent attendant levels stimulating factor-1 targeted through adherent precursor. Examination this steroid-sensitive precursor population demonstrates binding 125I-mannose bovine serum albumin spontaneously progressively increases with time in culture. Whereas macrophages cultured for 2 days express 3 X 10(4) mannose receptors/cell, number sites 7 10(4)/cell day 4. When precursors are exposed 1,25-(OH)2D3, an additional stepwise enhancement obtains time. Four culture results 1.6 10(5) a 100% increase compared control cells. Neither duration nor exposure alters KD which approximates 3-5 10(-9) ml-1. Finally, "specificity" D-mediated receptor established demonstrating does not alter 125I-alpha-thrombin marrow-derived precursors.