Hemoglobin switching in sheep. Isolation of the fetal gamma-globin gene and demonstration that the fetal gamma- and adult beta A-globin genes lie within eight kilobase segments of homologous DNA.
作者： P.J. Kretschmer , H.C. Coon , A. Davis , M. Harrison , A.W. Nienhuis
DOI: 10.1016/S0021-9258(19)69903-5
关键词:
摘要: Abstract A recombinant DNA library of sheep genomic fragments inserted into the bacteriophage vector, Charon 4A, was screened by plaque hybridization with a probe for gamma-globin gene sequences. Three clones containing overlapping segments DNA, total length which 25 kb, were identified; each included all or part same globin gene. Nucleotide sequencing substantial portions in one clone, lambda S gamma G31, and previously isolated recombinant, beta AG21, established that genes these recombinants encoded gamma- A-globin sheep, respectively. Features characteristic other species identified both sequence, ATAAAA, 30 nucleotides from presumed site initiation transcription, region "capping homology," two introns at positions corresponding to amino acids 29-30 103-104 polyadenylation AATAAA, 3' untranslated region. Electron microscopic analysis heteroduplexes formed between G31 AG21 revealed lie within homologous least 8 kb small regions nonhomology 5' genes.
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