Chiral separation of dansyl amino acids in capillary electrophoresis using mono-(3-methyl-imidazolium)-β-cyclodextrin chloride as selector

摘要: Enantioseparations of fourteen dansyl amino acids were achieved by using a positively-charged single-isomer beta-cyclodextrin, mono-(3-methyl-imidazolium)-beta-cyclodextrin chloride, as chiral selector. Separation parameters such buffer pH, selector concentration, separation temperature, and organic modifier investigated for the enantioseparation in order to achieve maximum possible resolution. Chiral was found be highly dependent on pH since degree protonation these can alter strength electrostatic interaction and/or inclusion complexation between each enantiomer In general, resolution enhanced at higher which indicates that carboxylate group analytes may interact with imidazolium cationic cyclodextrin. For most analytes, distinct enantioresolution obtained 8.0. Moreover, further improved careful tuning concentration (e.g. 10 mM), lower addition methanol. Enantioseparation standard mixture single run within 30 min.