Tissue Respiration and Mitochondrial Oxidative Phosphorylation of NaCl-Treated Pea Seedlings

作者: Avinoam Livne , Naomi Levin

DOI: 10.1104/PP.42.3.407

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摘要: The effect of sodium chloride added to root medium pea seedlings on respiratory activity tissue segments and isolated mitochondria was studied. Salinization enhances the respiration leaves about one-third a fresh weight, dry weight or protein basis. Roots stems show only 10 15% stimulation. onset increase in roughly parallels NaCl content decrease growth rate. At later stage elevated is apparent treated plants even though concentration reaches plateau osmotic adjustment being reached. Stimulation found both etiolated green plants. Experiments with DNP that simple uncoupling by salt not involved; control proportionally same.In accordance increased rates observed vivo, from salt-treated higher oxygen uptake several substrates. during long term distinct Since P/O ratios are affected NaCl, potential for oxidative phosphorylation salt-affected appears increase. It postulated this may lead changes ADP ATP content, turn, affect regulation metabolic pathways.

参考文章(13)
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
BRITTON CHANCE, G. R. WILLIAMS, A method for the localization of sites for oxidative phosphorylation. Nature. ,vol. 176, pp. 250- 254 ,(1955) , 10.1038/176250A0
Gene W. Miller, Harold J. Evans, The Influence of Salts on the Activity of Particulate Cytochrome Oxidase from Roots of Higher Plants. Plant Physiology. ,vol. 31, pp. 357- 364 ,(1956) , 10.1104/PP.31.5.357
James M. Lyons, T. A. Wheaton, Harlan K. Pratt, Relationship between the Physical Nature of Mitochondrial Membranes and Chilling Sensitivity in Plants Plant Physiology. ,vol. 39, pp. 262- 268 ,(1964) , 10.1104/PP.39.2.262
A. J. Hiatt, Harold J. Evans, Influence of Salts on Activity of Malic Dehydrogenase from Spinach Leaves. Plant Physiology. ,vol. 35, pp. 662- 672 ,(1960) , 10.1104/PP.35.5.662
H. E. Hayward, L. Bernstein, PLANT-GROWTH RELATIONSHIPS ON SALT-AFFECTED SOILS' Botanical Review. ,vol. 24, pp. 584- 635 ,(1958) , 10.1007/BF02872595
J. D. Verleur, Studies on the Isolation of Mitochondria from Potato Tuber Tissue Plant Physiology. ,vol. 40, pp. 1003- 1007 ,(1965) , 10.1104/PP.40.6.1003