Basic and Clinical Aspects of Sperm Comet Assay

摘要: Sperm DNA damage is associated with poorer assisted reproductive treatment (ART) outcomes including reduced fertilization rates, embryo quality, and pregnancy rates higher of spontaneous miscarriage childhood diseases. It shows promise as a more robust biomarker infertility than conventional semen parameters. Among the sperm testing methods, alkaline comet assay sensitive, reliable, powerful tool to detect even low levels within individual sperm. The present chapter provides an overview use in This includes need for standardization protocol its strengths weaknesses. Since often result increased oxidative stress male tract, primarily formed due imbalance between reactive oxygen species generation antioxidant depletion, novel addition measure oxidized bases explored. potential therapy protect against such also described. Finally, diagnostic prognostic values measures determining technology success are discussed.