Characterization of porin from Roseobacter denitrificans.
作者： Uwe Neumann , Elke Maier , Emile Schiltz , Jürgen Weckesser , Roland Benz
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摘要: Porin from Roseobacter denitrificans was isolated and purified to homogeneity. The pore characteristics this marine bacterium were compared those of its phylogenetically closely related freshwater bacteria Rhodobacter capsulatus 37b4, sphaeroides Rhodopseudomonas blastica. porin formed weakly cation-selective, general diffusion pores in lipid bilayer membranes. High transmembrane potentials caused channel closing steps that one or two thirds the initial on-steps indicating R. comprised three more less independent channels similar PhoE OmpC Escherichia coli capsulatus. Prediction secondary structure 36 N-terminal amino acid residues indicated β-strands porins 37b4 Differences single conductivities between show evolved are well adapted habitat.
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