The Effects of γ Radiation on Chondrogenic Development in Vitro

摘要: Gamma radiation (0.9-8.0 Gy) was used as a perturbing agent to study factors influencing in vitro chondrogenesis of embryonic chick limb bud cell culture. Chondrogenesis measured using number criteria, including (1) cartilage nodule production, (2) spectrophotometric determination the amount bound Alcian blue dye, and (3) computer-assisted analysis spatial distribution (area) density present individual micromass colonies. inhibited both proliferation dose- time-dependent fashion. Administration benzamide caused significant increase at 0.9 2.7 Gy, all doses. Cartilage production affected during first 2 days (prior 48 h) culture only, suggesting that chondrocytic commitment occurs this period. Cultures irradiated 72 h produced same nodules controls, but significantly less presumably because decreased numbers and/or synthesis products. Computer colonies provided data similar those collected spectrophotometrically, displayed advantages increased sensitivity variations, ability collect sets without having pool three or more colonies, long-term storage raw images for later analysis.