Control of Drosophila opsin gene expression by carotenoids and retinoic acid: northern and western analyses.

摘要: In the fly, thorough retinoid deprivation is possible, to optimize investigation of effects vitamin A metabolites and retinoic acid (RA) on visual development. Retinoids had been found control fly opsin gene transcription, though this finding was contested. Northern blots Drosophila heads showed that mRNA Rh1 (the predominant rhodopsin) high in replete controls, very low deprived flies, increased upon feeding carrot juice flies as early 1 hr. Expression ribosomal protein 49 [rp49] control) equal both replacement. Recovery such carotenoid replacement followed, barely detectable Western at 4 hr but conspicuous by 8 Alternative chromophore with yeast-glucose food yielded Northerns not rhodopsin, demonstrated blots, spectrophotometry electroretinogram (ERG). Rh1's resulted from rearing egg adult otherwise deprivational medium supplemented RA or beef brain-heart infusion. By comparing results these different media it concluded that: [1] affect transcription; [2] contradictory conclusions were which does eliminate all dependent factors could promoter. Preliminary evidence shows decreases two proteins relevant function: phospholipase C (PLC); binding (DRBP).