Functional Screening for the Discovery of New Extremophilic Enzymes

摘要: Enzymes produced by extremophiles offer an attractive solution to industrial biocatalysis since unlike most enzymes today, extremozymes are more stable and robust, therefore making them ideal candidates for applications. For industry, confirming the functionality of a biocatalyst under conditions particular process makes difference between obtaining or not required product. Although molecular approaches allow access genes interest in fast way, obtain large amounts functional proteins, it is possible sole use genomics. Additionally, these cannot discern properties such as: activity, pH temperature stability, inhibitors, only measure through approach. New technologies will need integrate approach into large-scale biotechnology implementing technological capacity analyse some intrinsic features search various enzymatic activities at same time. This chapter recent relevant advances discovery development extremophilic with special emphasis implementation screening strategies as well rapid efficient tools. Furthermore, group selected biotransformations be analysed context applicability context.