Identification of nuclear encoded precursor tRNAs within the mitochondrion of Trypanosoma brucei.
作者： K Hancock , A.J. LeBlanc , D Donze , S.L. Hajduk
DOI: 10.1016/S0021-9258(18)35931-3
关键词:
摘要: RNAs that function in mitochondria are typically encoded by the mitochondrial DNA. However, tRNAs of Trypanosoma brucei nuclear DNA and therefore must be imported into mitochondrion. It is becoming evident RNA import phylogenetically widespread essential for cellular processes, but virtually nothing known about mechanism import. We have identified characterized precursor T. brucei. The identification mitochondrially located clearly indicates as precursors. hybridize to cloned tRNA genes, label with [alpha-32P]CTP using yeast nucleotidyltransferase isolated via an endogenous nucleotidyltransferase-like activity, processed mature Escherichia coli RNase P. show extract contains P activity capable processing a prokaryotic well Precursors tRNA(Asn) tRNA(Leu) were detected on Northern blots RNA, 5' ends these primer extension analysis. structure significance within mitochondrion discussed.
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