Evidence for the presence of oxidatively modified low density lipoprotein in atherosclerotic lesions of rabbit and man.
作者： S Ylä-Herttuala , W Palinski , M E Rosenfeld , S Parthasarathy , T E Carew
DOI: 10.1172/JCI114271
关键词:
摘要: Three lines of evidence are presented that low density lipoproteins gently extracted from human and rabbit atherosclerotic lesions (lesion LDL) greatly resembles LDL has been oxidatively modified in vitro. First, lesion showed many the physical chemical properties oxidized LDL, differ those plasma LDL: higher electrophoretic mobility, a density, free cholesterol content, proportion sphingomyelin lysophosphatidylcholine phospholipid fraction. A number lower molecular weight fragments apo B were found similar to vitro LDL. Second, both intact some reacted Western blots with antisera recognize malondialdehyde-conjugated lysine 4-hydroxynonenal adducts, which LDL; normal intima no such reactivity. Third, shared biological compared produced much greater stimulation esterification was degraded more rapidly by macrophages. Degradation radiolabeled competitively inhibited unlabeled copper, polyinosinic acid malondialdehyde-LDL, but not native indicating uptake scavenger receptor(s). Finally, (but intimal or chemotactic for monocytes, as is These studies provide strong lesions, man rabbit, contain
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