The Elg1 Replication Factor C-like Complex Functions in PCNA Unloading during DNA Replication
作者： Takashi Kubota , Kohei Nishimura , Masato T. Kanemaki , Anne D. Donaldson
DOI: 10.1016/J.MOLCEL.2013.02.012
关键词:
摘要: Summary The ring-shaped complex PCNA coordinates DNA replication, encircling to act as a polymerase clamp and sliding platform recruit other replication proteins. is loaded onto by factor C, but it has been unknown how removed from when Okazaki fragments are completed or the fork terminates. Here we show that Elg1 C-like (Elg1-RLC) functions in unloading. Using an improved degron system without Elg1, accumulates on Saccharomyces cerevisiae chromatin during replication. accumulated can be in vivo switching expression. We find moreover treating with purified Elg1-RLC causes unloading in vitro. Our results demonstrate of both unmodified SUMOylated while genome instability elg1Δ mutant suggests timely critical for chromosome maintenance.
elsevier.com
sci-hub.se 参考文章(35)
Kohei Nishimura, Tatsuo Fukagawa, Haruhiko Takisawa, Tatsuo Kakimoto, Masato Kanemaki, An auxin-based degron system for the rapid depletion of proteins in nonplant cells Nature Methods. ,vol. 6, pp. 917- 922 ,(2009) , 10.1038/NMETH.1401
Efterpi Papouli, Shuhua Chen, Adelina A. Davies, Diana Huttner, Lumir Krejci, Patrick Sung, Helle D. Ulrich, Crosstalk between SUMO and ubiquitin on PCNA is mediated by recruitment of the helicase Srs2p. Molecular Cell. ,vol. 19, pp. 123- 133 ,(2005) , 10.1016/J.MOLCEL.2005.06.001
George-Lucian Moldovan, Boris Pfander, Stefan Jentsch, PCNA Controls Establishment of Sister Chromatid Cohesion during S Phase Molecular Cell. ,vol. 23, pp. 723- 732 ,(2006) , 10.1016/J.MOLCEL.2006.07.007
B. A. Kelch, D. L. Makino, M. O'Donnell, J. Kuriyan, How a DNA polymerase clamp loader opens a sliding clamp Science. ,vol. 334, pp. 1675- 1680 ,(2011) , 10.1126/SCIENCE.1211884
Takashi Kubota, Shin-ichiro Hiraga, Kayo Yamada, Angus I. Lamond, Anne D. Donaldson, Quantitative proteomic analysis of chromatin reveals that Ctf18 acts in the DNA replication checkpoint Molecular & Cellular Proteomics. ,vol. 10, ,(2011) , 10.1074/MCP.M110.005561
Pamela Kanellis, Roger Agyei, Daniel Durocher, Elg1 Forms an Alternative PCNA-Interacting RFC Complex Required to Maintain Genome Stability Current Biology. ,vol. 13, pp. 1583- 1595 ,(2003) , 10.1016/S0960-9822(03)00578-5
S. Ben-Aroya, A. Koren, B. Liefshitz, R. Steinlauf, M. Kupiec, ELG1, a yeast gene required for genome stability, forms a complex related to replication factor C Proceedings of the National Academy of Sciences of the United States of America. ,vol. 100, pp. 9906- 9911 ,(2003) , 10.1073/PNAS.1633757100
Anthony A. Armstrong, Firaz Mohideen, Christopher D. Lima, Recognition of SUMO-modified PCNA requires tandem receptor motifs in Srs2 Nature. ,vol. 483, pp. 59- 63 ,(2012) , 10.1038/NATURE10883
Oren Parnas, Adi Zipin-Roitman, Boris Pfander, Batia Liefshitz, Yuval Mazor, Shay Ben-Aroya, Stefan Jentsch, Martin Kupiec, Elg1, an alternative subunit of the RFC clamp loader, preferentially interacts with SUMOylated PCNA The EMBO Journal. ,vol. 29, pp. 2611- 2622 ,(2010) , 10.1038/EMBOJ.2010.128
Thomas R. Beattie, Stephen D. Bell, The role of the DNA sliding clamp in Okazaki fragment maturation in archaea and eukaryotes. Biochemical Society Transactions. ,vol. 39, pp. 70- 76 ,(2011) , 10.1042/BST0390070