Cytotoxic activity and cell cycle analysis of quinoline alkaloids isolated from Haplophyllum canaliculatum Boiss
作者： Pegah Varamini , Katayoun Javidnia , Mohammad Soltani , Ahmad Reza Mehdipour , Abbas Ghaderi
关键词:
摘要: Bioassay-guided fractionation of Haplophyllum canaliculatum Boiss. (Rutaceae) extract resulted in isolation five quinoline alkaloids: 7-isopentenyloxy-gamma-fagarine, atanine, skimmianine, flindersine and perfamine. This is the first these compounds from this endemic species. The antitumor activity isolates was evaluated against RAJI, Jurkat, KG-1a, HEP-2, MCF-7, HL-60 HL-60/MX1 tumor cell lines. highest cytotoxic effect observed on acute lymphoblastic leukemia 7-Isopentenyloxy-gamma-fagarine, skimmianine exhibited very high cytotoxicity RAJI line with IC(50) values 1.5, 14.5, 15.6 14.9 microg/mL, respectively atanine Jurkat 3.6, 9.3 11.5 respectively. 7-Isopentenyloxy-gamma-fagarine also highly MCF-7 (IC(50) = 15.5 microg/mL), while perfamine showed moderate to low cells. All alkaloids had KG-1a HEP-2. Investigation toxic potential a significantly higher HL-60/MX1, multidrug-resistant line, compared control etoposide (p < 0.05). In all experiments, peripheral blood mononuclear cells (PBMC) were used as for normal hematopoietic Flow cytometry analysis arrest cycle progression at sub-G1 phase lines dose-dependent manner. According computational analyses, similar trend could be indicative fact that may act through parallel mechanisms.
cabdirect.org
thieme-connect.de 参考文章(19)
S. S. Nazrullaev, I. A. Bessonova, Kh. S. Akhmedkhodzhaeva, Estrogenic Activity as a Function of Chemical Structure in Haplophyllum Quinoline Alkaloids Chemistry of Natural Compounds. ,vol. 37, pp. 551- 555 ,(2001) , 10.1023/A:1014873000974
H. Saglam, T. Gozler, B. Kivcak, B. Demirci, K. H. C. Baser, VOLATILE COMPOUNDS FROM Haplophyllum myrtifolium Chemistry of Natural Compounds. ,vol. 37, pp. 442- 444 ,(2001) , 10.1023/A:1014467225815
D. M. Razakova, I. A. Bessonova, S. Yu. Yunusov, Perfamine — A new alkaloid from the seeds of Haplophyllum perforatum Chemistry of Natural Compounds. ,vol. 11, pp. 828- 829 ,(1975) , 10.1007/BF00568495
W. Graydon Harker, D. Lynn Slade, Fred H. Drake, Ryan L. Parr, Mitoxantrone resistance in HL-60 leukemia cells: reduced nuclear topoisomerase II catalytic activity and drug-induced DNA cleavage in association with reduced expression of the topoisomerase II beta isoform. Biochemistry. ,vol. 30, pp. 9953- 9961 ,(1991) , 10.1021/BI00105A020
I. A. Bessonova, V. I. Akhmedzhanova, S. Yu. Yunusov, 7-Isopentenyloxy- γ -fagarine from Haplophyllum perforatum Chemistry of Natural Compounds. ,vol. 10, pp. 701- 702 ,(1974) , 10.1007/BF00567894
Olivia Jansen, V. Akhmedjanova, L. Angenot, G. Balansard, A. Chariot, E. Ollivier, M. Tits, M. Frédérich, Screening of 14 alkaloids isolated from Haplophyllum A. Juss. for their cytotoxic properties. Journal of Ethnopharmacology. ,vol. 105, pp. 241- 245 ,(2006) , 10.1016/J.JEP.2005.11.001
VI Akhmedzhanova, Kh A Rasulova, IA Bessonova, AS Shashkov, ND Abdullaev, Luc Angenot, None, Folipidine, a New Type Quinoline Alkaloid from Plants of the Haplophyllum Genus Chemistry of Natural Compounds. ,vol. 41, pp. 60- 64 ,(2005) , 10.1007/S10600-005-0075-1
Ih-Sheng Chen, Inn-Wha Tsai, Che-Ming Teng, Jih-Jung Chen, Ya-Ling Chang, Feng-Nien Ko, Matthias C. Lu, John M. Pezzuto, Pyranoquinoline alkaloids from Zanthoxylum simulans Phytochemistry. ,vol. 46, pp. 525- 529 ,(1997) , 10.1016/S0031-9422(97)00280-X
Günter Brader, Markus Bacher, Harald Greger, Otmar Hofer, Pyranoquinolones and acridones from Vepris bilocularis Phytochemistry. ,vol. 42, pp. 881- 884 ,(1996) , 10.1016/0031-9422(95)00922-1
Julien Fiot, Olivia Jansen, Valentina Akhmedjanova, Luc Angenot, Guy Balansard, Evelyne Ollivier, HPLC quantification of alkaloids from Haplophyllum extracts and comparison with their cytotoxic properties Phytochemical Analysis. ,vol. 17, pp. 365- 369 ,(2006) , 10.1002/PCA.927