Nutrient concentration-sensitive microorganism-based biosensor
作者： Carl Frederik Werner , Simone Groebel , Christoph Krumbe , Torsten Wagner , Thorsten Selmer
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摘要: This work presents a biosensor based on living cells immobilised light-addressable potentiometric sensor (LAPS). The set-up is sensitive to the concentrations of nutrients metabolised by bacteria, which results in an extracellular acidification. exemplarily demonstrated with glucose as nutrient and Escherichia coli microorganism. function this comparable enzyme-based field-effect when using diffusion limitation. With help addressability LAPS on-chip differential was developed. Thus, external influences such drift, temperature pH changes can be minimised.
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