Immunotherapeutic effects of T11TS/S-LFA3 against nitrosocompound mediated neural genotoxicity

摘要: Nitrosocompounds formed by the interaction of nitrites and secondary amines are neurotoxic in human different rodent species. Human exposure nitrosocompounds widespread affected modes like nitrite/nitrate preserved foods, beverages beer, stomach following uptake precursors nitrates, amines. The productions alkylating metabolites during breakdown causative agents for changes neural cells. An attempt has been made our lab to study effect nitrosocompound mediated toxicity gradual toxic effects these transform normal glial cells a neoplastic one. present indicated that transmembrane glycopeptide sheep red blood cell (SRBC), known as S-LFA3 or T11 target structure (T11TS) applied induced animals manifesting full grown intracranial malignancy can revert back tumor-bearing condition physiological state. Young Druckray rat both sexes aging 3-5 days were injected with N'-N'-ethyl nitrosourea (ENU) intraperitoneally (i.p.) at single dose 80 mg/kg body weight simulate neurotoxicity. 2-,4-, 6-, 8-, 10-month-old neonatal ENU sacrificed growth kinetics, functional immunological parameters receptor studies hint tumor development. In order determine immunomodulatory role T11TS, 7-month-old T11TS 0.41 weight, three consecutive doses an interval 6 maintaining control untreated age-matched rats control. All parameters, kinetic study, receptor-based FACS directly established immunomodulatory, anti-toxic anti-tumor property T11TS/S-LFA3. Finally, formation DNA ladder along FACS-based apoptosis clearly is potent apoptotic inducer