Development of alkylating agent-resistant human tumor cell lines.

摘要: Survival curves and dose escalation studies of four representative human tumor cell lines exposed to the various alkylating agents are presented. With HN2, at a level one log kill there was fivefold range in drug concentration required achieve this degree among lines, from 4.5 microM for SL6 lung adenocarcinoma 22 SW2 small-cell carcinoma. Four logs SCC-25 squamous carcinoma cells were killed by 100 CDDP; however, only about 500 CDDP. To G3361 melanoma 24 4-HC microM, approximately 16-fold difference. The L-PAM appeared be biphasic, with break microM. There threefold L-PAM, 60 line 18 line. 295 BCNU 120 2.5-fold maximally tolerated HN2 concentrations 1200 line, 48 times initial concentration, 300 16 concentration. highest CDDP, 1900 on other hand, 600 30 36 (1450 microM), whereas (720 microM). 1555 700 14 BCNU, 4200 1450 8 In all cases, least able tolerate exposure increasing agents. showed greatest tolerance agents.(ABSTRACT TRUNCATED AT 400 WORDS)