Down-regulation of Orai3 arrests cell-cycle progression and induces apoptosis in breast cancer cells but not in normal breast epithelial cells

摘要: Breast cancer (BC) is the leading in world terms of incidence and mortality women. However, mechanism by which BC develops remains largely unknown. The increase cytosolic free Ca(2+) can result different physiological changes including cell growth death. Orai isoforms are highly selective channels. In present study, we analyzed Orai3 expression normal cancerous breast tissue samples, its role MCF-7 MCF-10A mammary epithelial lines. We found that mRNAs was higher tissues cells than cells. Down-regulation siRNA inhibited proliferation arrested cycle at G1 phase. This phenomenon associated with a reduction CDKs 4/2 (cyclin-dependent kinases) cyclins E D1 an accumulation p21(Waf1/Cip1) (a cyclin-dependent kinase inhibitor) p53 tumor-suppressing protein). also involved survival. Furthermore, mediated entry contributed to intracellular calcium concentration ([Ca(2+)](i)). cells, silencing failed modify [Ca(2+)](i), proliferation, cell-cycle progression, (D1, E), (4, 2), expression. Our results provide strong evidence for significant effect on vitro show this induction apoptosis resistance. study highlights possible as therapeutic target therapy.