Genetic Identification of the Wild Form of Olive (Olea Europaea var. Sylvestris) Using Allele-Specific Real-Time PCR.

摘要: The wild-type of olive tree, Olea europaea var Sylvestris or oleaster, is the ancestor cultivated tree. Wild-type oil considered to be more nutritious with increased antioxidant activity compared common type (Olea L. Europaea). This has led having a much higher financial value. Thus, wild one most susceptible agricultural food products adulteration other oils lower nutritional and economical As olives have similar phenotypes, there need establish analytical methods distinguish two plant species. In this work, new method been developed which able (wild-type olive) from Europaea (cultivated olive). based, for first time, on genotyping, by allele-specific, real-time PCR, single nucleotide polymorphism (SNP) present in olives’ chloroplastic genomes. With proposed method, we were detect as little 1% content binary DNA mixtures