Continuous Coagulation of Milk using Immobilized Cells of Cynara Cardunculus

摘要: Plant rennets are used in Portugal for the small-scale manufacture of certain varieties cheese, namely “Serra” and “Serpa”. In traditional preparation these types cheese clotting stage is induced by immersing flowers native Compositae Cynara cardunculus or Silybum marianum milk vats. However, are, themselves, a potential source microbial contamination product. This problem could be overcome plant cell culture. Previous work demonstrated that vitro cultivated cells both species present activity (Fevereiro et al. 1986, Esquivel 1986), no being found supernatant (Fonseca 1987).