RNA‐Seq Read Alignments with PALMapper

摘要: Next-generation sequencing technologies have revolutionized genome and transcriptome sequencing. RNA-Seq experiments are able to generate huge amounts of sequence reads at a fraction the cost Sanger Reads produced by these relatively short error prone. To utilize such for reconstruction gene-structure identification, one needs be accurately align over intron boundaries. In this unit, we describe PALMapper, fast easy-to-use tool that is designed compute both unspliced spliced alignments millions reads. It combines efficient read mapper GenomeMapper with aligner QPALMA, which exploits read-quality information predictions splice sites improve alignment accuracy. The PALMapper package available as command-line running on Unix or Mac OS X systems through Web interface based Galaxy tools.