The molecular mechanisms and gene expression profiling for shikonin-induced apoptotic and necroptotic cell death in U937 cells
作者： Jin-Lan Piao , Zheng-Guo Cui , Yukihiro Furusawa , Kanwal Ahmed , Mati Ur Rehman
DOI: 10.1016/J.CBI.2013.06.011
关键词:
摘要: Shikonin (SHK), a natural naphthoquinone derived from the Chinese medical herb Lithospermum erythrorhizon, induces both apoptosis and necroptosis in several cancer cell lines. However, detailed molecular mechanisms involved initiation of death are still unclear. In present study, caspase-dependent was induced by SHK treatment at 1μM after 6h U937 cells, with increase DNA fragmentation, generation intracellular reactive oxygen species (ROS), fraction cells low mitochondrial membrane potential (MMP), expression BH3 only proteins Noxa tBid. Interestingly, caspase-independent also detected 10μM, observed as SYTOX® Green staining release lactate dehydrogenase (LDH). Necrostatin-1 (Nec-1) completely inhibited SHK-induced leakage LDH staining. Cell permeable exogenous glutathione (GSH) converted 10μM to apoptosis. Gene profiling revealed that 353 genes were found be significantly regulated 85 treatment, respectively. Among these genes, transcription factor 3 (ATF3) DNA-damage-inducible transcript (DDIT3) highly expressed while tumor necrosis (TNF) mainly increased treatment. These findings provide novel information for mechanism necroptosis.
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sci-hub.se 参考文章(40)
K S Sellins, J J Cohen, Gene induction by gamma-irradiation leads to DNA fragmentation in lymphocytes. Journal of Immunology. ,vol. 139, pp. 3199- 3206 ,(1987)
K.-J. SONG, Y. S. JANG, Y. A. LEE, K. A. KIM, S. K. LEE, M. H. SHIN, Reactive oxygen species-dependent necroptosis in Jurkat T cells induced by pathogenic free-living Naegleria fowleri. Parasite Immunology. ,vol. 33, pp. 390- 400 ,(2011) , 10.1111/J.1365-3024.2011.01297.X
L R Gooding, S M Laster, J G Wood, Tumor necrosis factor can induce both apoptic and necrotic forms of cell lysis. Journal of Immunology. ,vol. 141, pp. 2629- 2634 ,(1988)
Jee-Youn Kim, Yong-Jun Kim, Sun Lee, Jae-Hoon Park, BNip3 is a mediator of TNF-induced necrotic cell death Apoptosis. ,vol. 16, pp. 114- 126 ,(2011) , 10.1007/S10495-010-0550-4
Valeria Petronilli, Daniele Penzo, Luca Scorrano, Paolo Bernardi, Fabio Di Lisa, The Mitochondrial Permeability Transition, Release of Cytochromecand Cell Death Journal of Biological Chemistry. ,vol. 276, pp. 12030- 12034 ,(2001) , 10.1074/JBC.M010604200
YoungSik Cho, Sreerupa Challa, David Moquin, Ryan Genga, Tathagat Dutta Ray, Melissa Guildford, Francis Ka-Ming Chan, Phosphorylation-Driven Assembly of the RIP1-RIP3 Complex Regulates Programmed Necrosis and Virus-Induced Inflammation Cell. ,vol. 137, pp. 1112- 1123 ,(2009) , 10.1016/J.CELL.2009.05.037
H. Wajant, The Fas Signaling Pathway: More Than a Paradigm Science. ,vol. 296, pp. 1635- 1636 ,(2002) , 10.1126/SCIENCE.1071553
S-H Lee, J H Bahn, N C Whitlock, S J Baek, Activating transcription factor 2 (ATF2) controls tolfenamic acid-induced ATF3 expression via MAP kinase pathways Oncogene. ,vol. 29, pp. 5182- 5192 ,(2010) , 10.1038/ONC.2010.251
Karen M Hajra, J Rebecca Liu, Apoptosome dysfunction in human cancer Apoptosis. ,vol. 9, pp. 691- 704 ,(2004) , 10.1023/B:APPT.0000045786.98031.1D
Yoshiaki Tabuchi, Ichiro Takasaki, Takashi Kondo, Identification of genetic networks involved in the cell injury accompanying endoplasmic reticulum stress induced by bisphenol A in testicular Sertoli cells. Biochemical and Biophysical Research Communications. ,vol. 345, pp. 1044- 1050 ,(2006) , 10.1016/J.BBRC.2006.04.177