Expression and humoral immune response to hepatitis C virus using a plasmid DNA construct.
作者： Broor S , Vaishnav Y , Dar L , Seth P , Ray S
DOI: 10.1016/S0255-0857(21)03128-5
关键词:
摘要: PURPOSE: The objective of this study was to clone a c-DNA fragment hepatitis C virus in eukaryotic expression vector and measure the efficacy humoral immune responses mice inoculated with recombinant plasmid. This an attempt lay foundation for HCV nucleic acid vaccine development future. METHODS: A BK146, type 1b, sub-cloned into pMT3. HepG2 COS cells were transfected construct, named pMT3-BK146. mRNA proteins studied by reverse transcribed polymerase chain reaction, radio Immunoprecipitation (RIPA) immunofluorescence (IFA). DNA construct injected footpad BALB/c antibody response tested enzyme immunoassay indirect immunofluorescence. RESULTS: transiently plasmid pMT3-BK146 showed RT-PCR, RIPA when Balb/c able generate specific ELISA IFA. CONCLUSIONS: cloned express core protein. also elicit mice. can be initial step towards potential infection.
sci-hub.st 参考文章(26)
A Takamizawa, C Mori, I Fuke, S Manabe, S Murakami, J Fujita, E Onishi, T Andoh, I Yoshida, H Okayama, Structure and organization of the hepatitis C virus genome isolated from human carriers Journal of Virology. ,vol. 65, pp. 1105- 1113 ,(1991) , 10.1128/JVI.65.3.1105-1113.1991
J Dubuisson, H H Hsu, R C Cheung, H B Greenberg, D G Russell, C M Rice, Formation and intracellular localization of hepatitis C virus envelope glycoprotein complexes expressed by recombinant vaccinia and Sindbis viruses. Journal of Virology. ,vol. 68, pp. 6147- 6160 ,(1994) , 10.1128/JVI.68.10.6147-6160.1994
Guo-Jun Hu, Richard Y-H Wang, Dai-Shu Han, Harvey J Alter, J.Wai-Kuo Shih, Characterization of the humoral and cellular immune responses against hepatitis C virus core induced by DNA-based immunization. Vaccine. ,vol. 17, pp. 3160- 3170 ,(1999) , 10.1016/S0264-410X(99)00130-9
I. Saito, T. Miyamura, A. Ohbayashi, H. Harada, T. Katayama, S. Kikuchi, Y. Watanabe, S. Koi, M. Onji, Y. Ohta, Hepatitis C virus infection is associated with the development of hepatocellular carcinoma Proceedings of the National Academy of Sciences of the United States of America. ,vol. 87, pp. 6547- 6549 ,(1990) , 10.1073/PNAS.87.17.6547
T Saito, GJ Sherman, K Kurokohchi, ZP Guo, M Donets, MY Yu, JA Berzofsky, T Akatsuka, SM Feinstone, Plasmid DNA-based immunization for hepatitis C virus structural proteins: Immune responses in mice Gastroenterology. ,vol. 112, pp. 1321- 1330 ,(1997) , 10.1016/S0016-5085(97)70146-X
Harvey J. Alter, Robert H. Purcell, James W. Shih, Jacqueline C. Melpolder, Michael Houghton, Qui-Lim Choo, George Kuo, Detection of antibody to hepatitis C virus in prospectively followed transfusion recipients with acute and chronic non-A, non-B hepatitis The New England Journal of Medicine. ,vol. 321, pp. 1494- 1500 ,(1989) , 10.1056/NEJM198911303212202
K Tokushige, T Wakita, C Pachuk, D Moradpour, D B Weiner, V R Zurawski, J R Wands, Expression and immune response to hepatitis C virus core DNA-based vaccine constructs. Hepatology. ,vol. 24, pp. 14- 20 ,(1996) , 10.1002/HEP.510240104
J. Bukh, R. H. Purcell, R. H. Miller, Sequence analysis of the core gene of 14 hepatitis C virus genotypes. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 91, pp. 8239- 8243 ,(1994) , 10.1073/PNAS.91.17.8239
B. Wang, K. E. Ugen, V. Srikantan, M. G. Agadjanyan, K. Dang, Y. Refaeli, A. I. Sato, J. Boyer, W. V. Williams, D. B. Weiner, Gene inoculation generates immune responses against human immunodeficiency virus type 1. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 90, pp. 4156- 4160 ,(1993) , 10.1073/PNAS.90.9.4156
Jeffrey B Ulmer, John J Donnelly, Suezanne E Parker, Gary H Rhodes, Philip L Felgner, VJ Dwarki, Stanislaw H Gromkowski, R Randall Deck, Corrille M DeWitt, Arthur Friedman, Linda A Hawe, Karen R Leander, Douglas Martinez, Helen C Perry, John W Shiver, Donna L Montgomery, Margaret A Liu, Heterologous protection against influenza by injection of DNA encoding a viral protein Science. ,vol. 259, pp. 1745- 1749 ,(1993) , 10.1126/SCIENCE.8456302