Molecular cloning and characterization of Antheraea mylitta cytoplasmic polyhedrosis virus genome segment 9

摘要: Genome segment 9 of the 11-segment RNA genomes three cytoplasmic polyhedrosis virus (CPV) isolates from Antheraea mylitta (AmCPV), assamensis (AaCPV) and proylei (ApCPV) were converted to cDNA, cloned sequenced. In each case, this genome consists 1473 nucleotides with one long ORF 1035 bp encodes a protein 345 amino acids, termed NSP38, molecular mass 38 kDa. Secondary structure prediction showed presence nine α-helices in central terminal domains localized similarity RNA-binding motifs bluetongue infectious bursal disease polymerases. Nucleotide sequences 99·6% identical between these strains CPVs, but no was found any other nucleotide or sequence public databases. The AmCPV cDNA expressed as His-tagged fusion E. coli polyclonal antibody raised against purified protein. Immunoblot well immunofluorescence analysis anti-NSP38 that not present polyhedra uninfected cells AmCPV-infected host midgut cells. NSP38 insect soluble via baculovirus expression vector shown possess ability bind poly(rI)–(rC) agarose, which competitively removed by viral RNA. These results indicate is virus-infected non-structural By binding RNA, it may play role regulation genomic function packaging.