摘要: ABSTRACT SRPX2 (Sushi repeat-containing protein, X-linked 2) has recently emerged as a multifunctional protein that is involved in seizure disorders, angiogenesis and cellular adhesion. Here, we analyzed this biochemically. was secreted with highly post-translational modification. Chondroitinase ABC treatment completely decreased the molecular mass of purified to its predicted size, whereas heparitinase, keratanase hyaluroinidase did not. Secreted also detected using an anti-chondroitin sulfate antibody. These results indicate novel chondroitin proteoglycan (CSPG). Furthermore, binding assay revealed hepatocyte growth factor dose-dependently binds ligand–glycosaminoglycans interaction speculated be likely proteoglycans. Regarding architecture, sushi repeat modules similar four other CSPGs/lecticans; however, architecture seems quite different from lecticans. Taken together, found CSPG overexpressed gastrointestinal cancer cells. Our findings provide key glycobiological insight into cells demonstrate new member cancer-related family.
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