Comparison of different enzymeimmunoassays for assessment of adrenocortical activity in primates based on fecal analysis

摘要: Most studies published to date that used fecal glucocorticoid measurements assess adrenocortical activity in primate (and many nonprimate) species applied a specific cortisol or corticosterone assay. However, since these native glucocorticoids are virtually absent the feces of most vertebrates, including primates, validity this approach has recently been questioned. Therefore, overall aim present study was four enzymeimmunoassays (EIAs) using antibodies raised against cortisol, corticosterone, and reduced metabolites (two group-specific antibodies) for assessing metabolite (GCM) selected (marmoset, long-tailed macaque, Barbary chimpanzee, gorilla). Using physiological stimulation hypothalamo-pituitary-adrenocortical (HPA) axis by administering exogenous ACTH anesthesia, we demonstrated at least two assays detected predicted increase GCM levels response treatment each species. magnitude varied between species, no one assay applicable all While generally only limited suitability output, valuable those (according high-performance liquid chromatography (HPLC) analysis data) excreted clearly detectable amounts authentic into feces. In contrast, which feces, provided much stronger signal, also performed well other tested (except marmoset). Collectively, data suggest reliability given reflecting HPA primates depends on question. Although there is single system can be successfully across our have high potential cross-species application. Nevertheless, regardless GC antibody chosen, reinforces necessity appropriately validating respective before it used. Am. J. Primatol. 68:257–273, 2006. © 2006 Wiley-Liss, Inc.