Maternal microchimerism in peripheral blood in type 1 diabetes and pancreatic islet β cell microchimerism

摘要: Maternal cells have recently been found in the circulation and tissues of mothers' immune-competent children, including adult life, is referred to as maternal microchimerism (MMc). Whether MMc confers benefits during development or later life sometimes has adverse effects unknown. Type 1 diabetes (T1D) an autoimmune disease that primarily affects children young adults. To identify quantify MMc, we developed a panel quantitative PCR assays targeting nontransmitted, nonshared maternal-specific HLA alleles. was assayed peripheral blood from 172 individuals, 94 with T1D, 54 unaffected siblings, 24 unrelated healthy subjects. levels, expressed genome equivalent per 100,000 proband cells, were significantly higher T1D patients than siblings Medians ranges, respectively, 0.09 (0–530), 0 (0–153), (0–7.9). Differences between groups evident irrespective genotypes. However, for T1D-associated DQB1*0302-DRB1*04 haplotype, more often when haplotype paternally (70%) rather maternally transmitted (14%). In other studies, looked female islet β four male pancreases autopsies, one patient, employing FISH X Y chromosomes concomitant CD45 cell insulin staining. Female (presumed maternal) formed 0.39–0.96% total, whereas hematopoietic very rare. Thus, levels their contributes mother's progeny.