Rat guanidinoacetate methyltransferase: Mutation of amino acids within a common sequence motif of mammalian methyltransferase does not affect catalytic activity but alters proteolytic susceptibility
作者： Gomi Tomoharu , Tanihara Kiyomi , Date Takayasu , Fujioka Motoji
DOI: 10.1016/0020-711X(92)90182-Z
关键词:
摘要: Abstract 1. Manual alignment of amino acid sequences mammalian S-adenosylmethionine-dependent methyltransferases known sequence revealed the presence 2 homologous regions. 2. The region at C-terminal side is unique to methyltransferases, and in guanidinoacetate methyltransferase this occurs residues 159–165. 3. Mutagenesis 5 conserved did not affect catalytic activity but altered tryptic susceptibility Arg 20 .
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