Identification of a sequence-tagged site (STS) marker linked to a restorer gene for Ogura cytoplasmic male sterility in radish ( Raphanus sativus L.) by non-radioactive AFLP analysis
作者： Seiji Murayama , Toshiyuki Habuchi , Hiroshi Yamagishi , Toru Terachi
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摘要: To identify DNA markers linked to a fertility restorer (Rf) genefor Ogura cytoplasmic male sterility in radish (Raphanus sativus L.),a non-radioactive, amplified fragment length polymorphism (AFLP) analysiswas performed on bulked samples from male-sterile and male-fertileradishes. Ten male-fertile 10 plants selected arbitrarilyfrom an F2 population made by selfing of F1 plant crossbetween (`MS-Gensuke') (`Comet') plantwere used as material. Using 32 AFLP primer pairs, one fragment(AFLP190) which is specific the male-fertileF2 was identified. AFLP190 characterized molecularcloning nucleotide sequencing, converted sequence-taggedsite (STS) marker, STS190. A linkage analysis 126individuals two independent populations showed tight linkageof STS190 Rf gene. The rate recombination between themarker estimated be less than 1%, making STS1901.2 cM
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