In vitro generation of polysialylated cervical mucins by bacterial polysialyltransferases to counteract cytotoxicity of extracellular histones

摘要: Neutrophil extracellular traps (NET) are formed against pathogens. However, various diseases directly linked to this meshwork of DNA. The cytotoxic properties histones especially seem be an important trigger during these diseases. Furthermore, NET accumulation on implants is discussed result in impaired efficiency or failure, depending the category implant. Interestingly, mucins have been investigated as surface coatings potentially capable reducing neutrophil adhesion. Similarly, polysialic acid was shown inactivate histones. We wanted combine probability decrease adhesion neutrophils using with capability sialic polymers counteract histone-mediated cytotoxicity. To end, we elongate cervical bacterial polysialyltransferases. Subsequent cell-based experiments demonstrated activity elongated Thus, polysialylated may represent a novel component coat combat exaggerated formation.