The influence of time, storage temperature, and substrate age on potential soil enzyme activity in acidic forest soils using MUB-linked substrates and L-DOPA.

作者: Jared L. DeForest

DOI: 10.1016/J.SOILBIO.2009.02.029

关键词:

摘要: The purpose of this experiment was to evaluate whether soil storage and processing methods significantly influence measurements of potential in situ enzyme activity in acidic forest soils. More specifically, the objectives were to determine if:(1) duration and temperature of soil storage;(2) duration of soil slurry in buffer; and (3) age of model substrates significantly influence the activity of six commonly measured soil extracellular enzymes using methylumbelliferone (MUB)-linked substrates and l-dihydroxyphenylalanine …

参考文章(18)
Arthur L. Rosenthal, Abraham Saifer, Continuous UV monitoring of fluorogenic substrates Analytical Biochemistry. ,vol. 55, pp. 85- 92 ,(1973) , 10.1016/0003-2697(73)90292-3
HN Fernley, PG Walker, Kinetic behaviour of calf-intestinal alkaline phosphatase with 4-methylumbelliferyl phosphate. Biochemical Journal. ,vol. 97, pp. 95- 103 ,(1965) , 10.1042/BJ0970095
Yong Bok Lee, Nicola Lorenz, Linda Kincaid Dick, Richard P. Dick, Cold Storage and Pretreatment Incubation Effects on Soil Microbial Properties Soil Science Society of America Journal. ,vol. 71, pp. 1299- 1305 ,(2007) , 10.2136/SSSAJ2006.0245
P. R. Darrah, P. J. Harris, A fluorimetric method for measuring the activity of soil enzymes Plant and Soil. ,vol. 92, pp. 81- 88 ,(1986) , 10.1007/BF02372269
E. Kandeler, H. Gerber, Short-term assay of soil urease activity using colorimetric determination of ammonium Biology and Fertility of Soils. ,vol. 6, pp. 68- 72 ,(1988) , 10.1007/BF00257924
Jared L. DeForest, Donald R. Zak, Kurt S. Pregitzer, Andrew J. Burton, Atmospheric Nitrate Deposition, Microbial Community Composition, and Enzyme Activity in Northern Hardwood Forests Soil Science Society of America Journal. ,vol. 68, pp. 132- 138 ,(2004) , 10.2136/SSSAJ2004.1320
M.-C Marx, M Wood, S.C Jarvis, A microplate fluorimetric assay for the study of enzyme diversity in soils Soil Biology & Biochemistry. ,vol. 33, pp. 1633- 1640 ,(2001) , 10.1016/S0038-0717(01)00079-7
Anders R. Johnsen, Ole S. Jacobsen, A quick and sensitive method for the quantification of peroxidase activity of organic surface soil from forests Soil Biology & Biochemistry. ,vol. 40, pp. 814- 821 ,(2008) , 10.1016/J.SOILBIO.2007.10.017