Spontaneous and CRISPR/Cas9-induced mutation of the osmosensor histidine kinase of the canola pathogen Leptosphaeria maculans

摘要: The dicarboximide fungicide iprodione has been used to combat blackleg disease of canola (Brassica napus), caused by the fungus Leptosphaeria maculans. For example, in Australia was late 1990s but is no longer registered for use against disease, and therefore impact on L. maculans not investigated. Resistance emerged spontaneously under vitro conditions at high frequency. A basis this resistance mutations hos1 gene that encodes a predicted osmosensing histidine kinase. While loss homologous kinase some fungi deleterious effects growth pathogenicity, strains with mutated had reduced salt conditions, were still capable causing lesions B. napus. relative ease isolate mutants provided method develop then optimize CRISPR/Cas9 system disruptions maculans, species until now particularly difficult manipulate targeted disruptions. initially effective vitro, emerges easily these are able cause canola. This may explain limited efficacy field conditions. Iprodione resistance, such as through genes like hos1, provides an direction optimization disruption techniques.